Regulation of Clc-2 chloride channel by protein kinase C phosphorylation

Regulation of Clc-2 chloride channel by protein kinase C phosphorylation by Simeon Wong Download PDF EPUB FB2

Abstract. This article focuses on activators and inhibitors of ClC ClC-2 Cl − channels from mouse, rabbit, rat, and human have been studied extensively in recombinant form by whole-cell patch-clamp and in single-channel studies.

There are similarities in responses of these channels from different species to protons, ATP, lipids, and lubiprostone for the recombinant by: 1.

Chloride channels are proteins that control cell membrane transport of chloride ions and hence modulate intestinal fluid secretion. Chloride channel openers, such as lubiprost are currently under development for the treatment of patients with constipation, IBS-C, and postoperative activating the specific chloride channel CIC2 in cells lining the gut, lubiprostone increases.

Chloride channels are a superfamily of poorly understood ion channels specific for channels may conduct many different ions, but are named for chloride because its concentration in vivo is much higher than other anions.

Several families of voltage-gated channels and ligand-gated channels (e.g., the CaCC families) have been characterized in ro: IPR The ClC-2 channel was identified as a target of regulation by the M phase-specific cyclin-dependent kinase p34cdc2/cyclin B; the C terminus of ClC-2 is directly phosphorylated by p34cdc2/cyclin B.

As a result, ClC-2 channel activities are inhibited by p34cdc2/cyclin B in Xenopus oocytes with ClC-2 overexpression [ 80 ].Cited by: Chloride channel activity of ClC-2 is modified by the actin cytoskeleton Article (PDF Available) in Biochemical Journal Pt 3(Pt 3) January with 39 Reads How we measure 'reads'.

Chloride channel 3 (Clcn3) is a volume-sensitive channel, mainly participating in the regulation of cell fundamental volume, and P-glycoprotein (P-gp) acts as its modulator. Serine/threonine-protein kinase Sgk1 also known as serum and glucocorticoid-regulated kinase 1 is an enzyme that in humans is encoded by the SGK1 gene.

SGK1 belongs to a subfamily of serine/threonine kinases that is under acute transcriptional control by several stimuli, including serum and kinase is activated by insulin and growth factors via phosphatidylinositide-3 Aliases: SGK1, SGK, serum/glucocorticoid regulated.

An outwardly rectifying Cl- current, ICl,swell, is activated by swelling and mechanical stretch of cardiac myocytes and has emerged as an important regulator of cardiac function in both physiologic and pathophysiologic situations. ICl,swell modulates cardiac electrical activity, myocyte volume regulation, apoptosis, and perhaps ischemic by:   Jacob TJC, Civan MM.

Role of ion channels in aqueous humor formation. American Journal of Physiology. ; C– Kawasaki M, Uchida S, Monkawa T, Miyawaki A, Mikoshiba K, Marumo F, Sasaki S. Cloning and expression of a protein kinase C-regulated chloride channel abundantly expressed in rat brain neuronal cells.

by: Phosphorylation and functional regulation of ClC-2 chloride channels expressed in Xenopus oocytes by M cyclindependent protein kinase.

Physiol. – Cited by: 1. Recent evidence suggests that chloride channels are critical for cell proliferation, migration, and differentiation.

We examined the effects of transforming growth factor (TGF)-β1 on chloride channel expression and associations with human conjunctival fibroblast (HConF) biology. To investigate the potential role of chloride channel (CLC)-2 in migration, transition to myofibroblasts and Cited by: 4.

Vij N, Zeitlin PL () Regulation of the ClC-2 lung epithelial chloride channel by glycosylation of SP1. Am J Respir Cell Mol Biol – PubMed Google Scholar Vogt K, Mellor J, Tong G, Nicoll R () The actions of synaptically released zinc at hippocampal mossy fiber by: @article{osti_, title = {Crystallization and Preliminary X-ray Analysis of the Human Long Myosin Light-Chain Kinase 1-Specific Domain IgCAM3}, author = {W Vallen Graham and A Magis and K Bailey and J Turner and D Ostrov}, abstractNote = {Myosin light-chain kinase-dependent tight junction regulation is a critical event in inflammatory cytokine-induced increases in epithelial paracellular Author: W Vallen Graham.

The Red blood cell (RBC) membrane is naturally endowed with a variety of membrane transporters, mainly geared to optimize the respiratory function and to maintain cell homeostasis at minimal metabolic cost.

Therefore, to survive within a red blood cell, the malaria parasite must alter the permeability of the host’s plasma membrane by up-regulation of existing carriers or by creation of new Author: Serge L.

Thomas, Stéphane Egée. Angiotensin II (ANGII) plays a central role in the enhanced sodium reabsorption in early type 1 diabetes in man and in streptozotocin‐induced (STZ) diabetic rats. This study investigates the effect of.

Hu K, Duan D and Nattel S: Protein kinase C activates ATP-sensitive potassium current in human and rabbit ventricular myocytes. Circulation Research.

(IF: )(PMID) *Duan D: Characterization of a novel cardiac chloride channel and its regulation by cell volume and protein kinase C. Ph.D. Thesis. Not only is the activity of CFTR incrementally regulated by differential phosphorylation of the PKA consensus sites, protein kinase C-dependent phosphorylation may also modulate CFTR.

Interested readers are referred to several recent articles that have extensively reviewed this subject ( Cited by: A bicyclic fatty acid derived from prostaglandin E1 and a chloride channel activator with laxative activity. Upon intake, lubiprostone specifically binds to and activates the type 2 chloride channel (ClC-2) in the apical membrane of the gastrointestinal epithelium.

AMP-activated Protein Kinase Phosphorylates Golgi-specific Brefeldin A Resistance Factor 1 at Thr to Induce Disassembly of Golgi Apparatus Molecular Biology of the Cell, Vol. 16, No. Golgi structure in stress sensing and apoptosis Evidence for a Functional Interaction between the ClC-2 Chloride Channel and the Retrograde Motor.

Grubb, Barbara R., and Richard C. Boucher. Pathophysiology of Gene-Targeted Mouse Models for Cystic Fibrosis. Physiol. Rev. 79, Suppl.: S–S, — Mutations in the gene causing the fatal disease cystic fibrosis (CF) result in abnormal transport of several ions across a number of epithelial by: The CLH-3b channel is normally kept inactive by GCK-3 dependent phosphorylation of S and S, just downstream of its binding site (Denton et al., ; Falin et al., ).

Activation of CLH-3b is a result of dephosphorylation by the type 1 phosphatases GSP-1 and GSP-2 (Rutledge et al., ). Wang, Y. & Kotlikoff, M. Inactivation of calcium-activated chloride channels in smooth muscle by calcium/calmodulin-dependent protein kinase.

Proc. Natl Acad. Sci. Cited by: 1. The importance of chloride ions in cell physiology has not been fully recognized until recently, in spite of the fact that chloride (Cl-), together with bicarbonate, is the most abundant free anion in animal cells, and performs or determines fundamental biological functions in all tissues.

AS associates with the Na+,K+-ATPase and mediates the adenosine monophosphate-stimulated protein kinase-dependent regulation of sodium pump surface expression. Alves DS1, Farr GA, Seo-Mayer P, Caplan MJ. Expression of the chloride channel CLC-K in human airway epithelial cells. we identified expression of mRNA for ClC-2, ClC-3, ClC This book sheds new light on the physiology, molecular biology and pathophysiology of epithelial ion channels and transporters.

It combines the basic cellular models and functions by means of a compelling clinical perspective, addressing aspects from the laboratory bench to the bedside.

For patients with brain metastases, the association between miRNA and protein kinase Cα was significant (r =P = ), but that between miRNA and protein kinase Cα was not significant (r =P = ).

Another candidate gene identified in the 6p locus is the transporter 1 ATP-binding cassette sub-family B (TAP-1), a gene encoding the transporter associated with antigen processing that may be a susceptibility factor to JME.

22 CLCN2 encodes the voltage-gated chloride channel ClC-2, and it is regarded as a predisposing gene for by: If the address matches an existing account you will receive an email with instructions to retrieve your username.

Megalencephalic leukoencephalopathy with subcortical cysts protein-1 regulates epidermal growth factor receptor signaling in astrocytes and ERK1/2 kinase and PLCγ1 phosphorylation. K +-selective channel activated by the binding of Ca 2+ ions to calmodulin molecules constitutively associated with the channel protein (67,68).Cited by:   Proc.

Natl. Acad. Sci. USA – 36 Saley K., Smith R., Schaack J., Wilcox C. and Jentsch T. 15 Suzuki M. and Mizuno A. () A novel Cl channel related to () Alteration of GABA receptor function following gene Drosophila flightless locus. Biol. Chem – transfer of the CLC-2 chloride channel. The retinal pigment epithelium by Olaf Strauss.

This is supported by the pH sensitivity of basolateral ClC-2 Cl channel which increases its activity by a decrease of extracellular pH. () Regulation of L-type calcium channels by protein tyrosine kinase and protein kinase C in cultured rat and human retinal pigment epithelial cells.P Lopez-Lopez,C., Dietrich,M.O., Metzger,F., Loetscher,H., Torres-Aleman,I.

Disturbed cross talk between insulin-like growth factor I and AMP-activated protein kinase as a possible cause of vascular dysfunction in the amyloid precursor protein/presenilin 2 mouse model of Alzheimer’s disease.Lubiprostone activates ClC-2 chloride channels in epithelia.

It is approved for treatment of chronic idiopathic constipation in adults and constipation-predominate irritable bowel syndrome in women.